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According to various embodiments, there is provided a method for generating a reference clock signal, the method including discharging a capacitive element to a discharged state, when a reset signal has a predetermined reset state; charging the capacitive element from the discharged state to a first voltage, when a charge signal has a predetermined charge state; comparing the first voltage to a zero voltage, when a compare signal has a predetermined compare state; generating a second voltage based on the comparing of the first voltage to the zero voltage; generating a clock signal based on the second voltage, using an oscillator; and generating each of the reset signal, the charge signal and the compare signal, based on the clock signal.

A method of purifying a desired protein from a preparation includes (a) providing the preparation in a form having less than about 5% of chromatin residing in an original production medium, (b) contacting the preparation with a nonionic organic polymer and a salt, a concentration of nonionic organic polymer being sufficient to precipitate the desired protein or cause its accretion on a hydrophilic surface, or maintain it in a precipitated state or accreted on the hydrophilic surface, the salt concentration being sufficient to produce greater than physiological conductivity, and (c) contacting the preparation with at least one electropositive surface, optionally in the presence of a salt concentration sufficient to produce greater than physiological conductivity, the desired protein does not substantially adsorb to the at least one electropositive surface while not preventing adsorption of acidic contaminants to the at least one electropositive surface.

An optical sensing device is provided, including a first polarizer, a second polarizer, wherein the first polarizer and the second polarizer have respective transmission axes aligned in orthogonal directions, an SPR sensor arrangement including an SPR sensing surface, the SPR sensor arrangement arranged to receive an incident light beam passed through a polarizer to be reflected at the SPR sensing surface and transmitted through a second polarizer to provide a transmitted light beam, a detector arrangement configured to detect the transmitted light beam, the transmitted light beam including a sensing signal and a reference signal, and a processor electrically coupled to the detector arrangement, the processor configured to perform a subtraction operation between the sensing signal and the reference signal. The optical sensing is based on a differential measurement scheme. The subtraction between the sensing signal and the reference signal cancels the common path noise and enhances the sensor resolution.

Various aspects of the present invention relate to a peptide based biomaterial for visualization by SHG microscopy. In particular the invention relates to the use of short peptides as a non-linear optical (NLO) material for second harmonic generation (SHG) microscopy. A preferred short peptide comprises LIVAGK (LK6) and contains a non-polar aliphatic tail (with decreasing hydrophobicity) and a polar head; and can self-assemble into hydrogels; wherein which the peptide forms a tunable fibrous structure for in vitro and in vivo imaging applications and is suitable in disease diagnostics such as amyloidosis, including 1) neuro-degenerative amyloidosis, e.g. Alzheimer's (AD), Parkinson's, Huntington's (PD), 2) non-neuropathic localized amyloidosis such as in Type II Diabetes, and 3) systemic amyloidosis that occurs in multiple tissues, e.g. cataracts and lattice corneal dystrophy (LCD), as well as drug delivery and/or wound dressings.

There is provided a core-shell particle comprising a polymer conjugate encapsulating an active agent, wherein the polymer conjugate comprises a biocompatible polymer and a polyacidic polymer. There is also provided a process for forming the core-shell particle, a delivery vehicle comprising the core-shell particle and a method for delivering an active agent to a desired site.

The present invention refers to a multilayer barrier film capable of encapsulating a moisture and/or oxygen sensitive electronic or optoelectronic device, the barrier film comprises at least one nanostructured layer comprising reactive nanoparticles capable of interacting with moisture and/or oxygen, the reactive nanoparticles being distributed within a polymeric binder, and at least one ultraviolet light neutralizing layer comprising a material capable of absorbing ultraviolet light, thereby limiting the transmission of ultraviolet light through the barrier film.

The present invention relates to a composition for transfecting a cell with a genetic material comprising a first agent capable of directing the genetic material away from the acidic compartments in the cell and a second agent capable of stabilizing the microtubule or a network thereof. The invention also relates to the use of the composition in the manufacture of a medicament for treating a disease, a method for delivering a genetic material into a cell and a kit.

There is provided a hypermotor activity detection system, including: a video capture device configured to monitor a patient and obtain a video input; and a processing system configured to process the video input. The processing system includes: an optical flow module configured to carry out an optical flow analysis on a video input region determined from the video input, and further determine a flow field magnitude of the video input region; and a local motion reference module configured to obtain a local motion reference value. The local reference module includes: a division module; a sub-window flow module; a local motion magnitude module; and a local motion comparison module. Further, a corresponding method for detecting hypermotor activity is provided.

There is provided a nanoparticle-containing hydrogel comprising metal nanoparticles and at least one peptide. The nanoparticle-containing hydrogel may possess antibacterial activity. There is also provided a method for preparing the nanoparticle-containing hydrogel, and compositions and uses thereof.

A method of producing electronic plasmons by applying a bias to a molecular tunnel junction to excite plasmons, in which the molecular tunnel junction contains a top metallic electrode formed of a eutectic metal alloy and a metal oxide, a bottom metallic electrode formed of a transition metal, and a self-assembled monolayer formed of a plurality of organic molecules disposed between the top metallic electrode and the bottom metallic electrode. Also disclosed are a molecular tunnel junction for producing electronic plasmons and a method for preparing such a molecular tunnel junction.